Proteomics

Dataset Information

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Proteomic analysis of a precision knock-out Glycogen Synthase Kinase 3B model identifies new roles for GSK3B in colorectal cancer


ABSTRACT: Glycogen-specific kinase (GSK3β) is an integral regulator of the Wnt signalling pathway as well as many other diverse signalling pathways and processes. Dys-regulation of GSK3β is implicated in many different pathologies, including neurodegenerative disorders as well as many different tumour types. An important GSK3β mechanism of action is phosphorylation of substrates with consequent inhibition or degradation. In the context of tumour development, GSK3β has been shown to play both oncogenic and tumour suppressor roles, depending upon tissue, signalling environment or disease progression. Although multiple substrates of the GSK3β kinase have been identified, the wider protein networks within which protein-protein interaction networks and complexes within which GSK3β participates are not well known, and the consequences of these interactions not well understood. In this study LC-MS/MS expression analysis of knock-out GSK3β colorectal cancer cells is used as well as to understand the consequences on these interaction partners of GSK3β deletion from the system.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Rob Ewing  

LAB HEAD: Rob Ewing

PROVIDER: PXD014919 | Pride | 2021-10-07

REPOSITORIES: Pride

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