Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chinese giant salamander liver LC-MS/MS

ABSTRACT: Chinese giant salamander Andrias davidianus has strong tolerance to starvation. Here, a high-throughput proteomic analysis was carried out on liver samples obtained from the A. davidianus after 3, 7 and 11 months of fasting.s a result, the expression levels of 364 proteins were significantly changed in the fasted liver. This study provides insights into the molecular mechanisms underlying the metabolic response of A. davidianus liver to fasting.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Andrias Davidianus

TISSUE(S): Liver

SUBMITTER: Xiaofang Geng

LAB HEAD: Xiaofang Geng

PROVIDER: PXD014924 | Pride | 2020-01-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Differential Proteomic Analysis of Chinese Giant Salamander Liver in Response to Fasting.

Geng Xiaofang X Guo Jianlin J Zhang Lu L Sun Jiyao J Zang Xiayan X Qiao Zhigang Z Xu Cunshuan C

Frontiers in physiology 20200318

Chinese giant salamander <i>Andrias davidianus</i> has strong tolerance to starvation. Fasting triggers a complex array of adaptive metabolic responses, a process in which the liver plays a central role. Here, a high-throughput proteomic analysis was carried out on liver samples obtained from adult <i>A. davidianus</i> after 3, 7, and 11 months of fasting. As a result, the expression levels of 364 proteins were significantly changed in the fasted liver. Functional analysis demonstrated that the ...[more]

PMID: 32256382

Similar Datasets

BHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells

Project description:Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, DTMUV can also infect geese, chickens, house sparrows and replicate in in the brain, spleen, liver and kidneys of BALB/c mice, which poses public health concerns. So developing vaccines to combat DTMUV becomes urgent. Baby hamster kidney cell line (BHK-21) is usually employed to produce veterinary vaccine and DTMUV as well as other flaviviruses can be propagated in BHK-21 cells. However, information about mammalian host cell responses to DTMUV infection is limited. In this study, LC–MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells. We identified 192 differentially expressed cellular proteins including 11 upregulated proteins and 8 downregulated at 24 hpostinfection; 25 upregulated proteins and 151 downregulated at 48 h postinfection. Some of these proteins were involved in viral infection.

2017-05-23 | PXD005731 | Pride

Proteomic Analyses of Cysteine Redox in High-fat-fed and Fasted Mouse Livers: Implications for Liver Metabolic Homeostasis

Project description:Intensive oxidative stress occurs during high-fat-diet-induced hepatic fat deposition, suggesting a critical role for redox signaling in liver metabolism. Intriguingly, lines of evidence show that fasting could also result in redox profile changes, largely through reduced oxidant and/or increased anti-oxidant levels. However, a comprehensive landscape of redox-modified hepatic substrates is lacking, thus hindering our understanding of liver metabolic homeostasis. In this study, we employed a proteomic approach which combines iodoTMT and nanoLC-MS/MS to quantitatively probe the effects of high-fat-feeding and fasting on in vivo redox-based cysteine modifications. Collectively, we identified 1258 redox cysteine sites in 603 proteins and quantified 846 sites in 403 proteins.

2022-03-03 | PXD006612 | Pride

Liver proteomics identifies metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

Project description:We characterized the liver proteome of six Pekin ducks from pantothenic acid-deficient (PAD) group and the pantothenic acid-adequate (control, CON) group.

2022-10-14 | PXD026607 | Pride

Comparative proteomic analysis of root tubers of Rehmannia glutinosa at different growth stages

Project description:In this study, roots of Rehmannia glutinosa were used as experimental material, and three tuber roots of Rehmannia glutinosa in extension stage (I), expansion stage (E) and mature stage (M) were selected as samples, The iTRAQ quantitative proteomic technology combined with two-dimensional liquid chromatography and tandem mass spectrometry (2D-LC-MSMS) technology was used to construct the root tuber proteome database of Rehmannia glutinosa, the genes related to the growth and development of Rehmannia glutinosa root tuber were found.

2022-02-17 | PXD025914 | Pride

iTRAQ-Based Quantitative Proteomic Analysis of Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells

Project description:Viscum coloratum (Kom.) Nakai is a variety of biological activities of medicinal plants, and the active component such as polysaccharides or alkaloids, also proved to have the effect of anti-tumor, anti-virus, anti-radiation, anti-oxidation and anti-infection. In this study, we examined the inhibition of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cells growth in a dose-dependent manner using CCK-8 kit assay, and flow cytometry analysis showed that VCP2 delayed cell cycle in G1 phase and induced apoptosis in HepG2 cells which may due to overexpressed p21Wafl/Cip1 and Cyclin D and decreased expressions of Cyclin E and CDK4, and the upregulated Bad, Smac and Caspase-3 and the downregulated Bcl-XL and XIAP may be one of reasons for inducing apoptosis in VCP2-treated HepG2 cells, but these effects may be not obviously related to Bad. Using iTRAQ and 2D-LC-MSMS, 113 differentially expressed proteins identified in normal and VCP2-treated HepG2 cells. Among them, the expressions of 59 proteins were upregulation, and the expressions of 54 proteins were downregulation. GO, pathways and PPI of differentially expressed proteins further analyzed in polysaccharides-treated HepG2 cells by bioinformatic approach. These findings widen our understanding the anti-tumor mechanisms of mistletoe polysaccharide and provide new clues for screening potent responsive protein to polysaccharides.

2018-10-26 | PXD005323 | Pride

A 6-Month Systems Toxicology Inhalation/Cessation Study in ApoE-/- Mice to Investigate Cardiovascular and Respiratory Exposure Effects of the Modified-Risk Tobacco Products CHTP1.2 and THS2.2 Compared with the Effects of Conventional Cigarettes [Liver iTRAQ data].

Project description:Cigarette smoke (CS) causes adverse health effects that may occur shortly after smoking initiation and lead to the development of respiratory disease (chronic obstructive pulmonary disease), cardiovascular disease, and cancer. To reduce the risk of smokers developing smoking-related diseases, Philip Morris International is developing modified risk tobacco products (MRTP). Within a systems toxicology study, we integrated multi-omics measurements with classical endpoints to assess the effects in female ApoE-/- mice of six months of exposure to CS (at 29.9 µg nicotine/L) or to aerosols from one potential and one candidate MRTP (CHTP 1.2 and THS 2.2, respectively) at matched nicotine concentrations. The impact of cessation or switching to CHTP 1.2 after three months of CS exposure was also evaluated. This data set contains the results from the analysis of proteome changes in the liver using the iTRAQ® quantification approach.

2022-02-23 | PXD010874 | Pride

APP infected and healthy pig BALF iTRAQ-LC-MS/MS

Project description:Porcine pleuropneumonia caused by Actinobacilluspleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration of the mechanism. However, up to now, comparatively little information is available on the systemic and dynamic changes that occur in pig BALF in response to APP infection. This study used iTRAQ to identify differentially expressed proteins (DEPs) in pig BALF in response to APP infection.

2022-02-17 | PXD026983 | Pride

Rat motor and sensory nerve LC-MSMS

Project description:Peripheral nerve repair and functional recovery depend on the rate of nerve regeneration and the quality of target reinnervation. It is important to fully understand the cellular and molecular basis underlying the specificity of peripheral nerve regeneration, which means the achieving of respective correct pathfinding and accurate target reinnervation for regrowing motor and sensory axons. In this study, a quantitative proteomic technique, based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to profile the protein expression pattern between single motor and sensory nerves at 14 days after peripheral nerve transection. Among a total of 1259 proteins identified, 176 proteins showed the differential expressions between injured motor and sensory nerves. Quantitative real-time RT-PCR and Western blot analysis were applied to validate the proteomic data on representative differentially expressed proteins. Functional categorization indicated that differentially expressed proteins were linked to a diverse array of molecular functions, including axonogenesis, response to axon injury, tissue remodeling, axon ensheathment, cell proliferation and adhesion, vesicle-mediated transport, response to oxidative stress, internal signal cascade, and macromolecular complex assembly, which might play an essential role in peripheral motor and sensory nerve regeneration. Overall, we hope that the proteomic database obtained in this study could serve as a solid foundation for the comprehensive investigation of differentially expressed proteins between injured motor and sensory nerves and for the mechanism elucidation of the specificity of peripheral nerve regeneration.

2020-12-10 | PXD022097 | Pride

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in storability

Project description:iTRAQ-based comparative proteomic analysis on the critical node of viability loss in wheat seeds with obvious divergence in storability.

2021-09-25 | PXD025860 | Pride

APP infected and healthy Pig serum LC-MSMS

Project description:Porcine pleuropneumonia caused by Actinobacilluspleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration of the mechanism. However, up to now, comparatively little information is available on the systemic and dynamic changes that occur in pig serum in response to APP infection. This study used iTRAQ to identify differentially expressed proteins (DEPs) in pig serum in response to APP infection.

2021-09-09 | PXD017500 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data