Isotopic-labelled Clickable Glutathione to Quantify Protein S-Glutathionylation

ABSTRACT: Investigated protein glutathionylation in HL-1 cardiomyocyte cells in response to hydrogen peroxide using a clickable glutathione approach. After exposure, or not, to hydrogen peroxide the glutathionylated proteins with heavy or light azido glutathione were enriched and subjected to LC-MS/MS analysis followed by quantification.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Maheeshi Yapa Abeywardana  

LAB HEAD: Young-Hoon Ahn

PROVIDER: PXD014937 | Pride | 2021-09-08

REPOSITORIES: Pride