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Effect of ATG5 depletion on virus infection

ABSTRACT: Proteome analysis of wild-type and atg5-/- mouse embryonic fibroblasts infected with Japanese encephalitis virus

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Japanese Encephalitis Virus Group Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Manjula Kalia

LAB HEAD: Dr.Manjula Kalia

PROVIDER: PXD014986 | Pride | 2019-10-22

REPOSITORIES: Pride

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Quantitative Proteome Analysis of Atg5-Deficient Mouse Embryonic Fibroblasts Reveals the Range of the Autophagy-Modulated Basal Cellular Proteome.

Sharma Kiran Bala KB Sharma Manish M Aggarwal Suruchi S Yadav Amit Kumar AK Bhatnagar Shinjini S Vrati Sudhanshu S Kalia Manjula M

mSystems 20191105 6

Basal autophagy is crucial for maintenance of cellular homeostasis. ATG5 is an essential protein for autophagosome formation, and its depletion has been extensively used as a tool to disrupt autophagy. Here, we characterize the impact of Atg5 deficiency on the cellular proteome of mouse embryonic fibroblasts (MEFs). Using a tandem mass tagging (TMT)-based quantitative proteomics analysis, we observe that 14% of identified proteins show dysregulated levels in atg5-/- MEFs ...[more]

PMID: 31690592

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