Proteomics

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Phosphoproteomics of mouse cardiomyocytes reveals signalling pathways triggered by a vasodilator cryptide

ABSTRACT: Animal venoms are natural sources of bioactive molecules such as cryptides that consist in biological active peptides released after proteolytic cleavage of a peptide or protein precursor. The KPP is a cryptided derived from the c-terminal of an hypotensin (TsHpI) a peptide isolated from the venom of Tityus serrulatus scorpion. Previous studies of our group in rodent model demonstrated that KPP maintained the cardioprotective properties of its precursor. Nonetheless, the molecular machinery involved in the cellular response is poorly described to date. Thus, in order to characterize the signalling pathways triggered by KPP we incubate mouse cardiomyocytes with KPP during 5 or 30 min and explore the cell proteome and phosphoproteome by dimethyl labelling quantitation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart, Cardiac Muscle Cell

SUBMITTER: Thiago Verano-Braga  

LAB HEAD: Thiago Verano-Braga

PROVIDER: PXD015068 | Pride | 2020-07-06

REPOSITORIES: Pride

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Publications

Moving Pieces in a Cellular Puzzle: A Cryptic Peptide from the Scorpion Toxin Ts14 Activates AKT and ERK Signaling and Decreases Cardiac Myocyte Contractility via Dephosphorylation of Phospholamban.

Goméz-Mendoza Diana P DP   Lemos Rafael Pereira RP   Jesus Itamar C G ICG   Gorshkov Vladimir V   McKinnie Shaun M K SMK   Vederas John C JC   Kjeldsen Frank F   Guatimosim Silvia S   Santos Robson Augusto RA   Pimenta Adriano M C AMC   Verano-Braga Thiago T  

Journal of proteome research 20200709 8

Cryptic peptides (cryptides) are biologically active peptides formed after proteolysis of native precursors present in animal venoms, for example. Proteolysis is an overlooked post-translational modification that increases venom complexity. The tripeptide KPP (Lys-Pro-Pro) is a peptide encrypted in the C-terminus of Ts14-a 25-mer peptide from the venom of the <i>Tityus serrulatus</i> scorpion that has a positive impact on the cardiovascular system, inducing vasodilation and reducing arterial blo  ...[more]

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