Project description:Analysis of CPEB translational regulator target mRNAs Microarray analysis of mRNAs associated with polysomes in wild type (WT) and Cpeb1 KO MEFs
Project description:mRNAseq and proteomic data set of one week old WT (Chop wt/wt CkmmCre wt/wt Dars2 fl/fl), Chop KO (Chop ko/ko CkmmCre wt/wt Dars2 fl/fl), Dars2 KO (Chop wt/wt CkmmCre tg/wt Dars2 fl/fl) and DKO (Chop ko/ko CkmmCre tg/wt Dars2 fl/fl) mice
Project description:Comparison of kidneys of 4 different Pept2 KO individuals (GSE1585), pool of them and kidneys of WT individuals versus another WT individual (GSE1586). Keywords = Pept2 Keywords = Slc15a2 Keywords = kidney Keywords = KO Keywords = WT Keywords = pool Keywords = pooling Keywords = normal variatons Keywords = C57BL/6 Keywords = 129Sv Keywords: other
Project description:We generated NUP133 WT and KO podocytes to model human hereditary SRNS in vitro. A subtype of SRNS is caused by monogenetic mutations of the nuclear pore complex including NUP133. Immortalized human podocytes were genome edited applying the CRISPR/Cas9 technique and two independent NUP133 sgRNAs. Two monoclonal cell backgrounds were used to create two matching sets of NUP133 WT and KO cells (WT-1 versus KO-1 and WT-2 versus KO-2). Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis was performed in duplicate for each cell line. NUP133 KO podocytes exhibit vast transcriptional changes compared to WT cells.