Dataset Information

High-flow chromatography for reproducible and high-throughput quantification of proteomes

ABSTRACT: This study evaluates liquid-chromatography operated at 50 ul/min using 1 mm diameter columns coupled online to tandem mass spectrometry (LC-MS/MS) for proteome analysis. Data from >1,000 human cell line, tissue, body fluid, affinity- and phospho-proteomes demonstrate excellent chromatographic (<0.3 % CV) and quantitative (<7.5% CV) reproducibility. Combined with sample multiplexing by tandem mass tags, the system allows profiling of 11 proteomes in 16 hours to a depth of 8,000 proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF-X, Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Placenta, Blood Plasma, Cerebrospinal Fluid, Epithelial Cell, Cell Culture, Hela Cell

SUBMITTER: Yangyang Bian

LAB HEAD: Bernhard Kuster

PROVIDER: PXD015087 | Pride | 2019-12-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	02115_A10_P022097_S00_A00_R1.raw	Raw
	02115_A11_P022105_S00_A00_R1.raw	Raw
	02115_B10_P022098_S00_A00_R1.raw	Raw
	02115_B11_P022096_S00_A00_R2.raw	Raw
	02115_C10_P022099_S00_A00_R1.raw	Raw

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Publications

Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.

Bian Yangyang Y Zheng Runsheng R Bayer Florian P FP Wong Cassandra C Chang Yun-Chien YC Meng Chen C Zolg Daniel P DP Reinecke Maria M Zecha Jana J Wiechmann Svenja S Heinzlmeir Stephanie S Scherr Johannes J Hemmer Bernhard B Baynham Mike M Gingras Anne-Claude AC Boychenko Oleksandr O Kuster Bernhard B

Nature communications 20200109 1

Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. Here we show that micro-flow LC-MS/MS using a 1 × 150 mm column shows excellent reproducibility of chromatographic retention time (<0.3% coefficient of variation, CV) and protein quantification (<7.5% CV) using data from >2000 samples of human cell lines, tissues and body fluids. Deep proteome analysi ...[more]

PMID: 31919466

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