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PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM RHEUMATOID ARTHRITIS SYNOVIAL FLUID

ABSTRACT: Previously, extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators in recipient cells. However, mechanistic details are unclear and contaminants in EV enrichments might have compromised previous observations. We recently developed a novel, size exclusion chromatography (SEC)-based method of EV isolation capable of high-quality enrichments from human synovial fluid. Here, we employed this method in combination with high resolution mass spectrometry to accurately characterise the SF EV proteome and investigate contributions of SF EVs to inflammatory processes in RA.

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Rheumatoid Arthritis Disease Specific Synovial Fluid

DISEASE(S): Rheumatoid Arthritis

SUBMITTER: Laura Dagley

LAB HEAD: Ken C. Pang

PROVIDER: PXD015145 | Pride | 2021-04-01

REPOSITORIES: Pride

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Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.

Foers Andrew D AD Dagley Laura F LF Chatfield Simon S Webb Andrew I AI Cheng Lesley L Hill Andrew F AF Wicks Ian P IP Pang Ken C KC

Clinical & translational immunology 20201107 11

<h4>Objectives</h4>Extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators from recipient cells. We recently developed a size exclusion chromatography (SEC)-based method for EV isolation capable of high-quality enrichments from human SF. Here, we employed this method to accurately characterise the SF EV proteome and investigate potential contributions to inflammatory pathways in RA.<h4>Methods</h4>U ...[more]

PMID: 33204424

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8cabb52f-f5ec-4f58-8bff-c3c8a2140d47 - samples

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