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Cross-linking mass spectrometry of synapses purified from mouse brain

ABSTRACT: cross-linking experiments of three biologically independent replicates of hippocampal synaptosomes.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Fan Liu

LAB HEAD: Fan Liu

PROVIDER: PXD015160 | Pride | 2020-12-03

REPOSITORIES: Pride

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Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions.

Gonzalez-Lozano M A MA Koopmans F F Sullivan P F PF Protze J J Krause G G Verhage M M Li K W KW Liu F F Smit A B AB

Science advances 20200219 8

Synaptic transmission is the predominant form of communication in the brain. It requires functionally specialized molecular machineries constituted by thousands of interacting synaptic proteins. Here, we made use of recent advances in cross-linking mass spectrometry (XL-MS) in combination with biochemical and computational approaches to reveal the architecture and assembly of synaptic protein complexes from mouse brain hippocampus and cerebellum. We obtained 11,999 unique lysine-lysine cross-lin ...[more]

PMID: 32128395

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