Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Cell Culture
SUBMITTER: Alessandro Ori
LAB HEAD: Alessandro Ori
PROVIDER: PXD015170 | Pride | 2021-09-08
REPOSITORIES: Pride
Liu Huaize H Ding Jie J Köhnlein Karl K Urban Nadine N Ori Alessandro A Villavicencio-Lorini Pablo P Walentek Peter P Klotz Lars-Oliver LO Hollemann Thomas T Pfirrmann Thorsten T
Autophagy 20191203 9
The AMP-activated protein kinase (AMPK) regulates cellular energy homeostasis by sensing the metabolic status of the cell. AMPK is regulated by phosphorylation and dephosphorylation as a result of changing AMP/ATP levels and by removal of inhibitory ubiquitin residues by USP10. In this context, we identified the GID-complex, an evolutionarily conserved ubiquitin-ligase-complex (E3), as a negative regulator of AMPK activity. Our data show that the GID-complex targets AMPK for ubiquitination there ...[more]