Defining the interactome of CHD4 in fusion-positive rhabdomyosarcoma

ABSTRACT: The goal of this study was to explore in detail how the chromatin remodeler and NuRD subunit CHD4 controls the oncogenic signature of the tumor driver and fusion protein PAX3-FOXO1 in fusion-positive rhabdomyosarcoma. To this aim, we defined the interactome of CHD4 by LC-MS, identified its location in the genome by ChIP-seq, assessed its influence on DNA accessibility by DNase I hypersensitivity assays, and determined its target genes by RNA-seq.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Alveolar Sac

DISEASE(S): Alveolar Rhabdomyosarcoma

SUBMITTER: Fabian Frommelt  

LAB HEAD: Beat W. Schäfer

PROVIDER: PXD015231 | Pride | 2020-08-11

REPOSITORIES: Pride