Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture, Alveolar Sac
DISEASE(S): Alveolar Rhabdomyosarcoma
SUBMITTER: Fabian Frommelt
LAB HEAD: Beat W. Schäfer
PROVIDER: PXD015231 | Pride | 2020-08-11
REPOSITORIES: Pride
Marques Joana G JG Gryder Berkley E BE Pavlovic Blaz B Chung Yeonjoo Y Ngo Quy A QA Frommelt Fabian F Gstaiger Matthias M Song Young Y Benischke Katharina K Laubscher Dominik D Wachtel Marco M Khan Javed J Schäfer Beat W BW
eLife 20200803
The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex containing CHD4 localizes to super-enhancers where CHD4 generates a chromatin architecture permissive for the binding of the tumor driver and fusion protein PAX3-FOXO1, allowing downst ...[more]