Lysine fatty-acyl H3 peptide pulldown with HeLa SILAC nuclear extract

ABSTRACT: Histone H3 peptides with acyl modification on either K9 or K27 were used for peptide pull-down with stable isotope (light: K0R0, heavy: K8R10) labeled Hela nuclear extract. The acyl modifications are: acetyl (Ac), propionyl (Pro), Butanoyl (But), crotonyl (Cr), 3-hydroxy butanoyl (bHB), succinyl (Su), Pentanoyl (Vl), Hexanoyl (Xho) and Myristoyl (Myr). Each modified peptide is compared with an unmodified peptide with the same amino acid sequence. Both forward (unmodified-light, acyl-heavy) and reverse (unmodified-heavy, acyl-light) labeling and pull-down experiments were performed.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: John Coan  

LAB HEAD: Katrin Chua

PROVIDER: PXD015233 | Pride | 2019-10-02

REPOSITORIES: Pride