Fatty-acyl conjugated amino-hexyl agarose pulldown with HeLa SILAC whole cell extract

ABSTRACT: C10:0 and C14:0 fatty acids were conjugated onto agarose beads, and used for pull-down with stable isotope (light: K0R0, heavy: K8R10) labeled Hela whole cell extract. C2:0 conjugated beads were used as control. Both forward (C2:0-light, C10:0 or C14:0-heavy) and reverse (C2:0-heavy, C10:0 or C14:0-light) labeling and pull-down experiments were performed.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: John Coan  

LAB HEAD: Katrin Chua

PROVIDER: PXD015272 | Pride | 2019-10-02

REPOSITORIES: Pride