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Absolute quantification of proteins and histone modifications in Drosophila embryos

ABSTRACT: Mass spectrometry analysis for absolute and relative quantification of the nuclear proteome and of methyl-marks on selected lysine residues in histone H3 during two stages of Drosophila embryogenesis: 2-4 hr and 14-16 hr old.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Andreas Schmidt

LAB HEAD: Axel Imhof

PROVIDER: PXD015306 | Pride | 2023-02-01

REPOSITORIES: Pride

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Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation.

Bonnet Jacques J Lindeboom Rik G H RGH Pokrovsky Daniil D Stricker Georg G Çelik Muhammed Hasan MH Rupp Ralph A W RAW Gagneur Julien J Vermeulen Michiel M Imhof Axel A Müller Jürg J

Developmental cell 20191017 5

Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome and methyl marks on selected lysine residues in histone H3 during two stages of Drosophila embryogenesis. These analyses provide comprehensive information about the absolute copy number of several thousand proteins and reveal unexpected relationships between the abund ...[more]

PMID: 31630981

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