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Signaling proteins of a chemosensory pathway from sperm of Arbacia punctulata

ABSTRACT: Cilia and flagella serve as cellular antennae that translate sensory information into cellular responses. In sperm, a single chemoattractant molecule triggers signaling events that evoke a Ca2+ response and chemotactic steering. Key components of the chemotactic signaling pathway from sperm of the sea urchin Arbacia punctulata were investigated. Shotgun proteomics employing SDS-PAGE LC-MS/MS and MudPIT provided the proteome inventory of sperm flagella.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arbacia Punctulata

TISSUE(S): Sperm, Sperm Flagellum

SUBMITTER: Ansgar Poetsch

LAB HEAD: Ansgar Poetsch

PROVIDER: PXD015332 | Pride | 2019-12-11

REPOSITORIES: Pride

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Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation.

Trötschel Christian C Hamzeh Hussein H Alvarez Luis L Pascal René R Lavryk Fedir F Bönigk Wolfgang W Körschen Heinz G HG Müller Astrid A Poetsch Ansgar A Rennhack Andreas A Gui Long L Nicastro Daniela D Strünker Timo T Seifert Reinhard R Kaupp U Benjamin UB

The EMBO journal 20191227 4

Cilia serve as cellular antennae that translate sensory information into physiological responses. In the sperm flagellum, a single chemoattractant molecule can trigger a Ca<sup>2+</sup> rise that controls motility. The mechanisms underlying such ultra-sensitivity are ill-defined. Here, we determine by mass spectrometry the copy number of nineteen chemosensory signaling proteins in sperm flagella from the sea urchin Arbacia punctulata. Proteins are up to 1,000-fold more abundant than the free cel ...[more]

PMID: 31880004

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Signaling proteins of a chemosensory pathway from sperm of Arbacia punctulata

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