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Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome

ABSTRACT: In the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in Staphylococcus aureus. We discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. Overall, a comprehensive map of the bacterial cysteinome is obtained.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Stephan M. Hacker

LAB HEAD: Dr. Stephan M. Hacker

PROVIDER: PXD015457 | Pride | 2019-12-02

REPOSITORIES: Pride

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Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome.

Zanon Patrick R A PRA Lewald Lisa L Hacker Stephan M SM

Angewandte Chemie (International ed. in English) 20200107 7

Rapid development of bacterial resistance has led to an urgent need to find new druggable targets for antibiotics. In this context, residue-specific chemoproteomic approaches enable proteome-wide identification of binding sites for covalent inhibitors. Described here are easily synthesized isotopically labeled desthiobiotin azide (isoDTB) tags that shortened the chemoproteomic workflow and allowed an increased coverage of cysteines in bacterial systems. They were used to quantify 59 % of all cys ...[more]

PMID: 31782878

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