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Multiplexed quantitative proteomics of extracellular vesicles released by endMSCs (EV-endMSCs)

ABSTRACT: The extracellular vesicles released by endMSCs (EV-endMSCs)were characterized by multiplexedquantitative proteomics approach. We hypothesized that inflammatory priming of adult stem cells may contribute to increase their therapeutic effectiveness, thus, extracellular vesicles derived from IFN-γ-primed endMSCs (IFNγ/EV-endMSCs) were compared to control counterparts (n=3 patients).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Inmaculada Jorge

LAB HEAD: Javier G Casado

PROVIDER: PXD015465 | Pride | 2019-12-17

REPOSITORIES: Pride

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Unraveling the Molecular Signature of Extracellular Vesicles From Endometrial-Derived Mesenchymal Stem Cells: Potential Modulatory Effects and Therapeutic Applications.

Marinaro Federica F Gómez-Serrano María M Jorge Inmaculada I Silla-Castro Juan Carlos JC Vázquez Jesús J Sánchez-Margallo Francisco Miguel FM Blázquez Rebeca R López Esther E Álvarez Verónica V Casado Javier G JG

Frontiers in bioengineering and biotechnology 20191220

Endometrial-derived Mesenchymal Stem Cells (endMSCs) are involved in the regeneration and remodeling of human endometrium, being considered one of the most promising candidates for stem cell-based therapies. Their therapeutic effects have been found to be mediated by extracellular vesicles (EV-endMSCs) with pro-angiogenic, anti-apoptotic, and immunomodulatory effects. Based on that, the main goal of this study was to characterize the proteome and microRNAome of these EV-endMSCs by proteomics and ...[more]

PMID: 31921832

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Exposure to aerosol of an e-vapor device with MESH™ technology had a lower biological impact than exposure to cigarette smoke on human bronchial and alveolar culture models: an in vitro systems toxicology assessment.

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