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Pseudomonas aerugionsa PAO1 transcriptome cultured in MOPS Glycerol and MOPS Acetate

ABSTRACT: Drastic alterations in transcripts associated with central carbon metabolism and associatedprocesses have been repeatedly observed a common expression program as P. aeruginosa adapts to the cystic fibrosis lung when compared to standard laboratory conditions. However, we have limited knowledge of how well these transcriptomic changes correlate with actual alterations in metabolic flux; the rate of turnover of molecules through metabolic pathways. We have compared the proteome of Pseudomonas aeruginosa PAO1 during growth on the carbon sources acetate and glycerol.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Pseudomonas Aeruginosa Pao1

TISSUE(S): Bacterial Protein

SUBMITTER: Stephen Dolan

LAB HEAD: Stephen Kevin Dolan

PROVIDER: PXD015615 | Pride | 2020-02-17

REPOSITORIES: Pride

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Contextual Flexibility in Pseudomonas aeruginosa Central Carbon Metabolism during Growth in Single Carbon Sources.

Dolan Stephen K SK Kohlstedt Michael M Trigg Stephen S Vallejo Ramirez Pedro P Kaminski Clemens F CF Wittmann Christoph C Welch Martin M

mBio 20200317 2

<i>Pseudomonas aeruginosa</i> is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of <i>P. aeruginosa</i> appears to converge toward a common metabolic program as the organism adapts to the CF airway environment. However, we still have only a limited understanding of how these transcriptional changes impact metabolic flux at the systems level. To address this, ...[more]

PMID: 32184246

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