Translatome Hypoxia-Acidosis, TMT-pulse SILAC
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ABSTRACT: Cells were grown in light (R0K0) SILAC media (AthenaES) for 7 days, they were then incubated in light media for 24 hours in respective condition (normoxia neutral (NN) pH: 7.4; hypoxia neutral (HN)1% O2, pH: 7.4; hypoxia acidosis (HA), 1% O2, pH=6) and pulsed with heavy (R10K8) SILAC media (AthenaES) for 16 hr (TMT-pSILAC) following treatment.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
DISEASE(S): Brain Glioblastoma Multiforme
SUBMITTER: Nathan Balukoff
LAB HEAD: Stephen Lee
PROVIDER: PXD015643 | Pride | 2020-09-28
REPOSITORIES: Pride
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