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Intracellular proteins adsorbed on AuNPs during transcytosis and exocytosis

ABSTRACT: we set protein corona as the research object in this study, and focused on exploring the biological effects of intracellular protein corona (IPC) that formed on particle surface after transcytosis of nanomedicines. Considering the multi-functionalization characteristic, gold nanoparticles (AuNPs) were fabricated here as the model of nanomedicines. We established a dual-filtration proteomics strategy based on mass spectrometry (MS) to detect the composition and category of IPC after transcytosis. Based on the analysis on IPC proteins, we did achieve novel and significant findings.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Mengmeng Qin

LAB HEAD: Mengmeng Qin

PROVIDER: PXD015651 | Pride | 2020-01-20

REPOSITORIES: Pride

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Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Qin Mengmeng M Zhang Jian J Li Minghui M Yang Dan D Liu Dechun D Song Siyang S Fu Jijun J Zhang Hua H Dai Wenbing W Wang Xueqing X Wang Yiguang Y He Bing B Zhang Qiang Q

Theranostics 20200101 3

The merits of nanomedicines are significantly impacted by the surrounding biological environment. Similar to the protein corona generated on the surface of nanoparticles in the circulation system, the intracellular protein corona (IPC) might be formed on nanoparticles when transported inside the cells. However, little is known currently about the formation of IPC and its possible biological influence. <b>Methods</b>: Caco-2 cells, a classical epithelial cell line, were cultured in Transwell plat ...[more]

PMID: 31938061

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