Proteomics

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The structure and symmetry of the Radial Spoke protein complex in Chlamydomonas flagella

ABSTRACT: Chemical cross-linking coupled to mass spectrometry was used to study the organization of the Radial Spoke protein complex from Chlamydomonas. Experiments were performed by on-bead cross-linking of the immobilized complex with the amine-reactive cross-linking reagent disuccinimidyl suberate (DSS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Chlamydomonas Reinhardtii

SUBMITTER: Alexander Leitner  

LAB HEAD: Takashi Ishikawa

PROVIDER: PXD015658 | Pride | 2020-08-07

REPOSITORIES: Pride

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The structure and symmetry of the radial spoke protein complex in <i>Chlamydomonas</i> flagella.

Poghosyan Emiliya E   Iacovache Ioan I   Faltova Lenka L   Leitner Alexander A   Yang Pinfen P   Diener Dennis R DR   Aebersold Ruedi R   Zuber Benoit B   Ishikawa Takashi T  

Journal of cell science 20200821 16

The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions - a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM) analysis to generate a 3D structure of the whole radial spo  ...[more]

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