Proteomics

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Characterization of Integrator module INTS10-INTS13-INTS14 and its connection to the cleavage module Part I

ABSTRACT: Chemical cross-linking coupled to mass spectrometry was used to study the complex between the Integrator complex subunits INTS13 (Asunder) and INTS14. Cross-linking was performed using disuccinimidyl suberate (DSS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Alexander Leitner  

LAB HEAD: Stefanie Jonas

PROVIDER: PXD015682 | Pride | 2020-06-18

REPOSITORIES: Pride

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INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

Sabath Kevin K   Stäubli Melanie L ML   Marti Sabrina S   Leitner Alexander A   Moes Murielle M   Jonas Stefanie S  

Nature communications 20200709 1

The Integrator complex processes 3'-ends of spliceosomal small nuclear RNAs (snRNAs). Furthermore, it regulates transcription of protein coding genes by terminating transcription after unstable pausing. The molecular basis for Integrator's functions remains obscure. Here, we show that INTS10, Asunder/INTS13 and INTS14 form a separable, functional Integrator module. The structure of INTS13-INTS14 reveals a strongly entwined complex with a unique chain interlink. Unexpected structural homology to  ...[more]

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