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Identification of pre-miR155 binding proteins from macrophases treated with LPS or LPS + IL10

ABSTRACT: Identification of pre-miR155 binding proteins using bioinylated pre-miR155 oligos and streptavidin magnetic beads from macrophages that are treated with LPS or LPS and IL10.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Jenny Moon

LAB HEAD: Alice Mui

PROVIDER: PXD015720 | Pride | 2020-05-26

REPOSITORIES: Pride

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Interleukin-10 control of pre-miR155 maturation involves CELF2.

Yoon Jeff S J JSJ Wu Mike K MK Zhu Tian Hao TH Zhao Helen H Cheung Sylvia T ST Chamberlain Thomas C TC Mui Alice L-F AL

PloS one 20200423 4

The anti-inflammatory cytokine interleukin-10 (IL10) is essential for attenuating inflammatory responses, which includes reducing the expression of pro-inflammatory microRNA-155 (miR155) in lipopolysaccharide (LPS) activated macrophages. miR155 enhances the expression of pro-inflammatory cytokines such as TNFα and suppresses expression of anti-inflammatory molecules such as SHIP1 and SOCS1. We previously found that IL10 interfered with the maturation of pre-miR155 to miR155. To understand the me ...[more]

PMID: 32324763

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