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Reanalysis of NOD mouse insulin granule and secreted peptidome data

ABSTRACT: Data from a previous publication from our lab (Nature 2018 Aug; 560 (7716): 107-111) were reanalyzed by performing a multiround search against a Uniprot-Mouse database followed by an in silico database of hybrid insulin peptides.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Secretory Granule, Pancreatic Islet Beta Cell

DISEASE(S): Type 1 Diabetes Mellitus

SUBMITTER: Cheryl Lichti

LAB HEAD: Cheryl Lichti

PROVIDER: PXD015726 | Pride | 2020-03-11

REPOSITORIES: Pride

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The MHC-II peptidome of pancreatic islets identifies key features of autoimmune peptides.

Wan Xiaoxiao X Vomund Anthony N AN Peterson Orion J OJ Chervonsky Alexander V AV Lichti Cheryl F CF Unanue Emil R ER

Nature immunology 20200309 4

The nature of autoantigens that trigger autoimmune diseases has been much discussed, but direct biochemical identification is lacking for most. Addressing this question demands unbiased examination of the self-peptides displayed by a defined autoimmune major histocompatibility complex class II (MHC-II) molecule. Here, we examined the immunopeptidome of the pancreatic islets in non-obese diabetic mice, which spontaneously develop autoimmune diabetes based on the I-A<sup>g7</sup> variant of MHC-II ...[more]

PMID: 32152506

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