Dataset Information

Protein phosphorylation networks in spargana of Spirometra erinaceieuropaei revealed by phosphoproteomic analysis

ABSTRACT: Background: Sparganosis caused by Spirometra erinaceieuropaei spargana is a zoonotic parasitic infection which has been reported in many countries, such as China, Japan, Thailand and Korea, as well as the Europe and United States. This parasite biological and clinical significances, genome, and transcriptome analysis have previously been investigated, but its phosphoproteomes have not been reported. Here, we investigated global and site-specific phosphoproteome profiling of the spargana. Results: 3228 phosphopeptides and 3461 phosphorylation sites were identified from 1758 spargana proteins. The annotated phosphoproteins were involved in the various biological mechanisms, which included the cellular, metabolic and single-organism processes. Additionally, functional enrichment of phosphopepetides in Gene Ontology analysis suggested that most spargana phosphoproteins were related to the cytoskeleton cellular compartment, the signaling molecular function, and a variety of biological processes, including a molecular function regulator, guanyl-nucleotide exchange factor activity, protein kinase activities, and calcium ion binding. The highly enriched pathway of phosphorylation proteins included the phosphatidylinositol signaling system, phagosome, endocytosis, inositol phosphate metabolism, terpenoid backbone biosynthesis, and peroxisome pathways. Domain analysis of phosphopeptides identified EF-hand domain and pleckstrin homology domain among the important domains. Conclusions: This study provides the first global phosphoproteomic analysis in the spargana. The reported dataset will shed light on future understanding of zoonotic parasite.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Spirometra Erinaceieuropaei

SUBMITTER: Yisong Liu

LAB HEAD: Wei Liu

PROVIDER: PXD015753 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

Protein phosphorylation networks in spargana of Spirometra erinaceieuropaei revealed by phosphoproteomic analysis.

Liu Wei W Tang Hailin H Abuzeid Asmaa M I AMI Tan Lei L Wang Aibing A Wan Xueping X Zhang Haoji H Liu Yisong Y Li Guoqing G

Parasites & vectors 20200513 1

<h4>Background</h4>Sparganosis caused by Spirometra erinaceieuropaei spargana is a zoonotic parasitic infection that has been reported in many countries, including China, Japan, Thailand and Korea, as well as European countries and the USA. The biological and clinical significance of the parasite have previously been reported. Although the genomic and transcriptomic analysis of S. erinaceieuropaei provided insightful views about the development and pathogenesis of this species, little knowledge ...[more]

PMID: 32404185

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Project description:All above ground organs of higher plants are ultimately derived from specialized organogenic structures termed shoot apical meristems (SAMs). The SAM exhibits distinctive structural organization, marked by cell layering. Maize SAMs are comprised of two cell layers, L1 (single cell layered tunica) and L2 (corpus). To identify genes required for layer-specific functions intact maize SAMs were fixed, embedded in paraffin and sectioned. L1 and L2 cells were isolated from these sections via laser capture microdissection (LCM). RNA was isolated from six biological replications of L1 and L2, amplified and hybridized to microarrays spotted with ~37,000 maize cDNA clones. This experiment identified ~700 ESTs that are preferentially expressed in the L1 or the L2 (P <0.001). The L1-up-regulated ESTs included ZmOCL1 and ZmOCL4, which are known to exhibit L1-specific expression in the maize SAM. The L2-up-regulated ESTs included KNOTTED1, whose transcripts are known to accumulate in the L2 but not in the L1 of the maize SAM. Differentially expressed ESTs included genes involved in transcription, signal transduction, transport and metabolism, many of which are novel candidates that are required for layer-specific functions in the maize SAM. Several L1-up-regulated ESTs were annotated as yabby family genes or basic helix-loop-helix transcription factor-like genes, which have not previously been reported as having layer-specific expression in the SAM. Novel WW domain-containing genes (WW genes) were identified in this study. The WW domain mediates protein-protein interactions, often with signal transduction components. These WW genes were substantially up-regulated in the L1 relative to the L2. Keywords: Cell Type Comparison An experimental aim is to identify genes that are differentially expressed in distinct histological cell layers of maize SAM by comparing the transcript accumulation between L1 (single cell layered tunica) and L2 (corpus) using cDNA microarrays that have over 37,000 informative spots from maize.

Dataset Information

Protein phosphorylation networks in spargana of Spirometra erinaceieuropaei revealed by phosphoproteomic analysis

Publications

Protein phosphorylation networks in spargana of Spirometra erinaceieuropaei revealed by phosphoproteomic analysis.

Similar Datasets

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