Proteomics

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A census of RNA/protein complexes in a model Gram-positive bacterium reveals exonuclease-mediated sRNA activation in competence regulation

ABSTRACT: 22 total proteome samples from S. pneumoniae wild type resolved on a glycerol gradient resulting in 20 fractions and a pellet fraction. A lysate sample is given as input control.

INSTRUMENT(S): LTQ Orbitrap Velos, Orbitrap Fusion

ORGANISM(S): Streptococcus Pneumoniae Tigr4

SUBMITTER: Jens Hör  

LAB HEAD: Jörg Vogel

PROVIDER: PXD015835 | Pride | 2020-04-08

REPOSITORIES: Pride

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Grad-seq in a Gram-positive bacterium reveals exonucleolytic sRNA activation in competence control.

Hör Jens J   Garriss Geneviève G   Di Giorgio Silvia S   Hack Lisa-Marie LM   Vanselow Jens T JT   Förstner Konrad U KU   Schlosser Andreas A   Henriques-Normark Birgitta B   Vogel Jörg J  

The EMBO journal 20200330 9

RNA-protein interactions are the crucial basis for many steps of bacterial gene expression, including post-transcriptional control by small regulatory RNAs (sRNAs). In stark contrast to recent progress in the analysis of Gram-negative bacteria, knowledge about RNA-protein complexes in Gram-positive species remains scarce. Here, we used the Grad-seq approach to draft a comprehensive landscape of such complexes in Streptococcus pneumoniae, in total determining the sedimentation profiles of ~ 88% o  ...[more]

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