Dataset Information

In planta bacterial multi-omics illuminates regulatory principles underlying plant-pathogen interactions

ABSTRACT: Gene regulation of bacterial pathogens in the host is not comprehensively understood due to the difficulty in analyzing genome-wide mRNA and protein expression of bacteria during infection. Here, we jointly analyzed transcriptome and proteome of a foliar bacterial pathogen in plants. Bacterial transcriptome changes can explain to a large extent their proteome changes in resistant and susceptible plants. However, a part of bacterial type III secretion system was suppressed by plant immunity preferentially at the protein level. Also, gene co-expression analysis uncovered previously unknown gene regulatory modules underlying bacterial virulence. Collectively, integrated in planta bacterial omics provides molecular insights into multiple layers of bacterial gene regulation that contributes to virulence and roles of plant immunity in controlling it.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Sara Christina Stolze

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD015839 | Pride | 2020-04-30

REPOSITORIES: Pride

ACCESS DATA

Publications

Multidimensional gene regulatory landscape of a bacterial pathogen in plants.

Nobori Tatsuya T Wang Yiming Y Wu Jingni J Stolze Sara Christina SC Tsuda Yayoi Y Finkemeier Iris I Nakagami Hirofumi H Tsuda Kenichi K

Nature plants 20200615 7

Understanding the gene regulation of plant pathogens is crucial for pest control and thus global food security. An integrated understanding of bacterial gene regulation in the host is dependent on multi-omic datasets, but these are largely lacking. Here, we simultaneously characterized the transcriptome and proteome of a bacterial pathogen in plants. We found a number of bacterial processes affected by plant immunity at the transcriptome and proteome levels. For instance, salicylic acid-mediated ...[more]

PMID: 32541952

Dataset Information

In planta bacterial multi-omics illuminates regulatory principles underlying plant-pathogen interactions

Publications

Multidimensional gene regulatory landscape of a bacterial pathogen in plants.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Similar Datasets

Proteomic response of the early divergent liverwort Marchantia polymorpha during infection with the oomycete pathogen Phytophthora palmivora
2019-06-24 | PXD012076 | Pride

The proteomic landscape of patient-derived CD4+ T cells in recent-onset type 1 diabetes
2017-11-30 | PXD006223 | Pride

Data-independent acquisition method for ubiquitinome analysis reveals non-degrative regulation of circadian biology
2020-11-20 | PXD019854 | Pride

Proteome dynamcis in biobanked horse peripheral blood derived lymphocytes (PBL) with induced autoimmune uveitis
2017-08-31 | PXD005580 | Pride

Spatiotemporal proteomic profiling of Huntington's disease mice reveals widespread loss of protein function
2017-11-24 | PXD004973 | Pride

Discovery and characterization of a new class of O-linking oligosaccharyltransferase from bacteria in the Moraxellaceae family
2022-10-13 | PXD033468 | Pride

Transcriptomic and proteomic landscape of SARS-CoV-2 liver tropism
2022-04-02 | PXD022789 | Pride

Transcription factor Msn2 dependent gene expression in two entomopathogen fungi, Beauveria bassiana and Metarhizium robertsii under thermal and oxidaitve stresses
2015-02-01 | E-GEOD-40765 | biostudies-arrayexpress

Comparative proteomic analysis of microvescicoles and exosomes isolated from urine of medullary sponge kidney disease (MSK) versus Autosomal dominant polycystic kidney disease (ADPKD) patients.
2019-11-25 | PXD011283 | Pride