Protease-resistant streptavidin for interaction proteomics

ABSTRACT: Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to different biotinylated biomolecules (DNA, protein and lipid) result in >100 streptavidin contamination and better coverage of both the target and its protein interactors without the need for sample pre-fractionation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Gianluca Sigismondo  

LAB HEAD: Jeroen Krijgsveld

PROVIDER: PXD015858 | Pride | 2025-04-08

REPOSITORIES: Pride