Dataset Information

Dog platelets proteome by label-free quantiative LC-MS/MS analysis

ABSTRACT: Proteomic analyses of the proteins secreted from dog platelets using label-free quantitationworkflow.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Blood Platelet

SUBMITTER: Sheng Zhang

LAB HEAD: Sheng Zhang

PROVIDER: PXD015864 | Pride | 2019-10-29

REPOSITORIES: Pride

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Publications

Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).

Cremer Signe E SE Catalfamo James L JL Goggs Robert R Seemann Stefan E SE Kristensen Annemarie T AT Brooks Marjory B MB

PloS one 20191113 11

Domestic dogs share the same environment as humans, and they represent a valuable animal model to study naturally-occurring human disease. Platelet proteomics holds promise for the discovery of biomarkers that capture the contribution of platelets to the pathophysiology of many disease states, however, canine platelet proteomic studies are lacking. Our study objectives were to establish a protocol for proteomic identification and quantification of the thrombin-activated canine platelet secretome ...[more]

PMID: 31721811

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Project description:In sickle cell disease, ischemia-reperfusion injury and intravascular hemolysis produce endothelial dysfunction and vasculopathy characterized by reduced nitric oxide (NO) and arginine bioavailability. Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, suggesting that pathological platelet activation may contribute to sickle cell disease vasculopathy. Studies were therefore undertaken to examine transcriptional signaling pathways in platelets that may be dysregulated in sickle cell disease. We demonstrate and validate here the feasibility of comparative platelet transcriptome studies on clinical samples from single donors, by the application of RNA amplification followed by microarray-based analysis of 54,000 probe sets. Data mining an existing microarray database, we identified 220 highly abundant genes in platelets and a subset of 72 relatively platelet-specific genes, defined by more than 10-fold increased expression compared to the median of other cell types in the database with amplified transcripts. The highly abundant platelet transcripts found in the current study included 82% or 70% of platelet abundant genes identified in two previous gene expression studies on non-amplified mRNA from pooled or apheresis samples, respectively. On comparing the platelet gene expression profiles in 18 patients with sickle cell disease in steady state to 12 African American controls, at a 3-fold cut-off and 5% false discovery rate, we identified ~100 differentially expressed genes, including multiple genes involved in arginine metabolism and redox homeostasis. Further characterization of these pathways using real time PCR and biochemical assays revealed increased arginase II expression and activity and decreased platelet polyamine levels. These studies suggest a potential pathogenic role for platelet arginase and altered arginine and polyamine metabolism in sickle cell disease and provide a novel framework for the study of disease-specific platelet biology. Experiment Overall Design: There are 18 sickle cell samples and 12 control samples from healthy African American volunteers.

Dataset Information

Dog platelets proteome by label-free quantiative LC-MS/MS analysis

Publications

Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).

Similar Datasets

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