Label free quantitative (LFQ) proteomic analysis of mouse lymphatic endothelial cells and primary mouse dendritic cells in response to IFNgamma treatment in presence or absence of 3-HKA

ABSTRACT: To explore the cellular pathways and the molecular functions affected by a novel biogenic amine, (3-HKA), we performed a label free quantitative (LFQ) proteomic analysis of mouse lymphatic endothelial cells (LEC), and primary dendritic cells, treated with IFNgammaplus or minus 3-HKA. Biological triplicates, of total cell lysates, were fractionated by one-dimensional gel electrophoresis (1DEF) and the “in gel” tryptic derived peptides analyzed by nano-LC-ESI-MS/MS on a Q Exactive HF quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). LFQ analysis highlighted that 3-HKA downregulated many of the inflammatory pathways, more notably JAK/STAT1 and NFkB, associated with IFNgamma activation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Endothelial Cell Of Lymphatic Vessel, Lymphatic Endothelial Cell, Dendritic Cell

SUBMITTER: Cristina Clement  

LAB HEAD: Laura Santambrogio

PROVIDER: PXD015865 | Pride | 2021-07-28

REPOSITORIES: Pride