Cystatin D-C26 immunocomplex characterization in mastocytosis

ABSTRACT: Mastocytosis is a myeloproliferative neoplasm characterized by infiltration of clonally derived mast cells in different tissues. According to mast cells localization, it is possible to discriminate cutaneous mastocytosis (CM) from systemic mastocytosis (SM), the latter involving at least an extracutaneous organ, like bone marrow, liver, spleen and gastrointestinal tract. Some of the SM patient can develop also cutaneous lesions (SM+C). Disease classification is often tricky. Oral cavity is commonly involved in the symptomatology. With the aim to highlight possible qualitative/quantitative modifications of the salivary proteome associated to the different forms of the disease, we investigated the protein profiles of CM, SM, and SM+C patients, and healthy controls, by integrated top-down and bottom-up integrated platforms. Into this project we focused also to the characterization of an immune multi-protein complex occurring in whole saliva and associated to cystatin D-C26 variant. This multi-protein complex with a high molecular weight (>250 KDa) included proteins and peptides involved in inflammatory processes and in the innate and acquired responses, events impaired in the mastocytosis patients. By using a combined immunoproteomics/gel-based proteomics/high-resolution MSMS analysis we investigated the qualitative/quantitative variations of the cystatin D-C26 complex (SIC-D) in different forms of mastocytosis and in healthy controls.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Mastocytosis

SUBMITTER: Tiziana Cabras  

LAB HEAD: Tiziana Cabras

PROVIDER: PXD015866 | Pride | 2026-10-07

REPOSITORIES: Pride