Dataset Information

A Non-interventional Clinical Trial Assessing Immune Responses After Radiofrequency Ablation of Liver Metastases From Colorectal Cancer

ABSTRACT: HLA ligandome analysis of colorectal adenocarcinoma tissues obtained from liver metastases (mCRC) as well as adjacent non-malignant liver tissues was performed to characterize the natural HLA class I and class II presented ligands on both mCRC and adjacent liver tissue.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver, Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Lena Mühlenbruch

LAB HEAD: Hans-Georg Rammensee

PROVIDER: PXD015947 | Pride | 2019-12-11

REPOSITORIES: Pride

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Publications

A Non-interventional Clinical Trial Assessing Immune Responses After Radiofrequency Ablation of Liver Metastases From Colorectal Cancer.

Löffler Markus W MW Nussbaum Bianca B Jäger Günter G Jurmeister Philipp S PS Budczies Jan J Pereira Philippe L PL Clasen Stephan S Kowalewski Daniel J DJ Mühlenbruch Lena L Königsrainer Ingmar I Beckert Stefan S Ladurner Ruth R Wagner Silvia S Bullinger Florian F Gross Thorben H TH Schroeder Christopher C Sipos Bence B Königsrainer Alfred A Stevanović Stefan S Denkert Carsten C Rammensee Hans-Georg HG Gouttefangeas Cécile C Haen Sebastian P SP

Frontiers in immunology 20191119

<b>Background:</b> Radiofrequency ablation (RFA) is an established treatment option for malignancies located in the liver. RFA-induced irreversible coagulation necrosis leads to the release of danger signals and cellular content. Hence, RFA may constitute an endogenous <i>in situ</i> tumor vaccination, stimulating innate and adaptive immune responses, including tumor-antigen specific T cells. This may explain a phenomenon termed abscopal effect, namely tumor regression in untreated lesions evide ...[more]

PMID: 31803175

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Project description:Biomaterials play an increasing role in clinical applications and regenerative medicine. Modern biomaterials should imitate the function of damaged tissue without triggering an immune response, initiate self-regeneration of the body and gradually degrade after implantation. The immune system is now well recognized to play a major role in influencing the biocompatibility of implanted medical devices. To develop a better understanding of the effects of biomaterials on the immune response, we have developed a highly sensitive novel test system capable of examining changes in the immune system by biomaterial. Here, we evaluate for the first time the immunopeptidome, a highly sensitive system that reflects cancer transformation, virus or drug influences and passes these changes directly to T cells, as a test system to examine the effects of contact with materials. Since monocytes are one of the first immune cells reacting to biomaterials, we have tested the influence of different materials - stainless steel, aluminum, zinc, high-density polyethylene, polyurethane films containing zinc diethyldithiocarbamate, copper, and zinc sulphate - on the monocytic THP-1 cell line. Material-associated peptides were identified after stimulation with all material types examined. The magnitude of induced changes in the immunopeptidome after the stimulation appears comparable to that of bacterial lipopolysaccharides (LPS). The source proteins of many detected peptides are associated with cytotoxicity, fibrosis, autoimmunity, inflammation and cellular stress. Considering all tested materials, it was found that the LPS-induced cytotoxicity-, inflammation- and cellular stress-associated HLA class I peptides were mainly induced by aluminum and HLA class II peptides mainly by stainless steel. These findings provide some of the first insights into the effects on the immunopeptidome by biomaterials. A more thorough understanding of these effects may enable the design of more biocompatible implant materials using in vitro models in future. This may be achieved through developing a deeper understanding of possible immune responses induced by biomaterials such as fibrosis, inflammation, cytotoxicity, and autoimmune reactions.

Dataset Information

A Non-interventional Clinical Trial Assessing Immune Responses After Radiofrequency Ablation of Liver Metastases From Colorectal Cancer

Publications

A Non-interventional Clinical Trial Assessing Immune Responses After Radiofrequency Ablation of Liver Metastases From Colorectal Cancer.

Similar Datasets

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