Dataset Information

Secretome of Acinetobacter baumannii KAB strains

ABSTRACT: We analyzed the extracellular proteome of colistin-resistant Korean Acinetobacter baumannii (KAB) strains to identify proteome profiles that can be used to characterize extensively drug-resistant KAB strains.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Acinetobacter Baumannii

SUBMITTER: Sang-Yeop Lee

LAB HEAD: Sang-Yeop Lee

PROVIDER: PXD015959 | Pride | 2020-05-27

REPOSITORIES: Pride

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Analysis of the Extracellular Proteome of Colistin-Resistant Korean Acinetobacter baumannii Strains.

Lee Sang-Yeop SY Yun Sung Ho SH Lee Hayoung H Yi Yoon-Sun YS Park Edmond Changkyun EC Kim Wooyoung W Kim Hye-Yeon HY Lee Je Chul JC Kim Gun-Hwa GH Kim Seung Il SI

ACS omega 20200311 11

We analyzed the extracellular proteome of colistin-resistant Korean Acinetobacter baumannii (KAB) strains to identify proteome profiles that can be used to characterize extensively drug-resistant KAB strains. Four colistin-resistant KAB strains with colistin resistance associated with point mutations in pmrB and pmrC genes were analyzed. Analysis of the extracellular proteome of these strains revealed the presence of 506 induced common proteins, which were hence considered a ...[more]

PMID: 32226849

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Project description:The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).

			Action	DRS
	A19606_SEC_1.mgf	Mgf
	A19606_SEC_1.mzid.gz	Mzid
	A19606_SEC_1.pride.mgf.gz	Mgf
	A19606_SEC_1.pride.mztab.gz	Mztab
	A19606_SEC_1_01.raw	Raw

Dataset Information

Secretome of Acinetobacter baumannii KAB strains

Dataset's files

Publications

Analysis of the Extracellular Proteome of Colistin-Resistant Korean <i>Acinetobacter baumannii</i> Strains.

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