LFQ-MS analysis of nucleosome pulldown experiments with symmetric and asymmetric nucleosomes

ABSTRACT: Recombinant mononucleosomes were generated carrying H3K4me3 and H3K27me3 in symmetric or asymmetric monovalent or asymmetric bivalent conformation, immobilized on beads and subjected to pulldown assays with mouse ESC nuclear extract. Binding was analyzed by quantitative LFQ-MS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Embryonic Stem Cell

SUBMITTER: Philipp Voigt  

LAB HEAD: Philipp Voigt

PROVIDER: PXD015990 | Pride | 2025-01-02

REPOSITORIES: Pride