Name: U2OS LC MSMS upon siRNA-eIF4A3 or 5nM ActD treatment.
Keywords: U2os lc msms upon sirna-eif4a3 or 5nm actd treatment.

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U2OS LC MSMS upon siRNA-eIF4A3 or 5nM ActD treatment.

ABSTRACT: Proteome analysis in U2OS cells treated with siRNA against eIf4A3 or 5nM Actinomycin D.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Osteosarcoma

SUBMITTER: Dimitrios Kanellis

LAB HEAD: Jiri Bartek

PROVIDER: PXD016005 | Pride | 2021-09-08

REPOSITORIES: Pride

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The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output.

Kanellis Dimitris C DC Espinoza Jaime A JA Zisi Asimina A Sakkas Elpidoforos E Bartkova Jirina J Katsori Anna-Maria AM Boström Johan J Dyrskjøt Lars L Broholm Helle H Altun Mikael M Elsässer Simon J SJ Lindström Mikael S MS Bartek Jiri J

Science advances 20210804 32

Eukaryotic initiation factor 4A-III (eIF4A3), a core helicase component of the exon junction complex, is essential for splicing, mRNA trafficking, and nonsense-mediated decay processes emerging as targets in cancer therapy. Here, we unravel eIF4A3's tumor-promoting function by demonstrating its role in ribosome biogenesis (RiBi) and p53 (de)regulation. Mechanistically, eIF4A3 resides in nucleoli within the small subunit processome and regulates rRNA processing via R-loop clearance. <i>EIF4A3</i> ...[more]

PMID: 34348895

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