Mass spectrometry proteomics detecting interaction proteins of Kindlin-2

ABSTRACT: We aim to find out the Kindlin-2 interaction proteins. We transfected plasmids Flag or Flag-Kindlin-2 to HKC cell lines and then used Flag-M2 beads to do the coimmunoprecipitation experiments and followed by NuPAGE Tris-Acetate Mini Gels detection and stained by Coomassie brilliant blue. Compared the two groups,and then we will find the proteins interact with Kindlin-2.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Jiagui Song  

LAB HEAD: Hongquan Zhang

PROVIDER: PXD016008 | Pride | 2019-12-16

REPOSITORIES: Pride