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Pronucleotide probe for in vivo imaging of protein AMPylation.

ABSTRACT: Protein AMPylation is a prevalent posttranslational modification in human cells involved in the regulation of unfolded protein response and neural development. Here we present a novel pro-N6azA probe suitable for in vivo imaging and chemical proteomics profilling of AMPylated proteins. The pro-N6azA probe provides stable fluorescent labelling in living cells accesible by straightforward strain-promoted azide-alkyne click reaction. Application of this probe may contribute significantly to the analysis of protein AMPylation during various metabolic processes including neurodevelopment and unfolded protein response.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Pavel Kielkowski

LAB HEAD: Stepahn A. Sieber

PROVIDER: PXD016011 | Pride | 2019-12-10

REPOSITORIES: Pride

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A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation.

Kielkowski Pavel P Buchsbaum Isabel Y IY Becker Tobias T Bach Kathrin K Cappello Silvia S Sieber Stephan A SA

Chembiochem : a European journal of chemical biology 20200206 9

Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells. ...[more]

PMID: 32027064

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