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Proteomics exploration of the human RPL3 interactome

ABSTRACT: Exploring the interactome of human RPL3 by MS-based proteomics.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Magnus Jakobsson

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD016055 | Pride | 2021-09-08

REPOSITORIES: Pride

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Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function.

Małecki Jędrzej M JM Odonohue Marie-Francoise MF Kim Yeji Y Jakobsson Magnus E ME Gessa Luca L Pinto Rita R Wu Jie J Davydova Erna E Moen Anders A Olsen Jesper V JV Thiede Bernd B Gleizes Pierre-Emmanuel PE Leidel Sebastian A SA Falnes Pål Ø PØ

Nucleic acids research 20210401 6

Protein methylation occurs primarily on lysine and arginine, but also on some other residues, such as histidine. METTL18 is the last uncharacterized member of a group of human methyltransferases (MTases) that mainly exert lysine methylation, and here we set out to elucidate its function. We found METTL18 to be a nuclear protein that contains a functional nuclear localization signal and accumulates in nucleoli. Recombinant METTL18 methylated a single protein in nuclear extracts and in isolated ri ...[more]

PMID: 33693809

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