Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Trypanosoma Cruzi Strain Cl Brener Leishmania Tarentolae
SUBMITTER: Lauriane Kuhn
LAB HEAD: Yaser Hashem
PROVIDER: PXD016063 | Pride | 2020-03-25
REPOSITORIES: Pride
Bochler Anthony A Querido Jailson Brito JB Prilepskaja Terezie T Soufari Heddy H Simonetti Angelita A Del Cistia Mayara Lucia ML Kuhn Lauriane L Ribeiro Aline Rimoldi AR Valášek Leoš Shivaya LS Hashem Yaser Y
Cell reports 20201201 12
Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA<sub>i</sub><sup>Met</sup> and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Tryp ...[more]