Name: Phosphorylation sites of PTPN22 treated by Calyculin A in 293T cells
Keywords: Ptpn22 phosphorylation

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Phosphorylation sites of PTPN22 treated by Calyculin A in 293T cells

ABSTRACT: Phosphorylation sites of PTPN22 induced by Calyculin A treatment were identified by LC-MS

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Shen Yang

LAB HEAD: Nunzio Bottini

PROVIDER: PXD016103 | Pride | 2021-09-08

REPOSITORIES: Pride

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PTPN22 phosphorylation acts as a molecular rheostat for the inhibition of TCR signaling.

Yang Shen S Svensson Mattias N D MND Harder Nathaniel H O NHO Hsieh Wan-Chen WC Santelli Eugenio E Kiosses William B WB Moresco James J JJ Yates John R JR King Charles C CC Liu Lin L Stanford Stephanie M SM Bottini Nunzio N

Science signaling 20200317 623

The hematopoietic-specific protein tyrosine phosphatase nonreceptor type 22 (PTPN22) is encoded by a major autoimmunity risk gene. PTPN22 inhibits T cell activation by dephosphorylating substrates involved in proximal T cell receptor (TCR) signaling. Here, we found by mass spectrometry that PTPN22 was phosphorylated at Ser<sup>751</sup> by PKCα in Jurkat and primary human T cells activated with phorbol ester/ionomycin or antibodies against CD3/CD28. The phosphorylation of PTPN22 at Ser<sup>751</ ...[more]

PMID: 32184287

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