Comparison of the total liver proteomes of postnatal-day-7 mice with liver-specific UBA3 deficiency and their littermate controls
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ABSTRACT: The total liver proteomes of postnatal-day-7 mice with liver-specific UBA3 deficiency and their littermate controls were compared by two-dimensional (2-D) electrophoresis mapping. 25 protein spots were obviously changed in their expression upon UBA3 deficiency. These 25 protein spots were excised from 2-D gels. After in-gel digestion, the tryptic digests were subjected to mass spectrometric analysis by LC–MS/MS, followed by database searching. Here, we identified ETFA expression in spot 14, which was decreased in UBA3-deficient conditions.
INSTRUMENT(S): Synapt MS
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Liver
SUBMITTER: Jiyan Zhang
LAB HEAD: Zhang jiyan
PROVIDER: PXD016111 | Pride | 2020-01-07
REPOSITORIES: Pride
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