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Immunopeptidome analysis of 4T1 murine breast cancer cells.

ABSTRACT: This study aimed to identify Tumor Associated Antigens (TAA)s by Immunoaffinity purification of MHC peptides and LCMS analysis, verifying their immunogenicity and testing their tumor control potential in a highly aggressive preclinical model of triple-negative breast cancer in mice. Among the MHC peptides thus identified, an Endogenous Retroviral (ERV) peptide was also found, and this peptide, as well as several other peptides from TAAs, were shown to possess tumor control potential in a cancer vaccine setting in mice, by restricting tumor growth compared to controls.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Breast, Breast Cancer Cell Line, Breast Cancer Cell

DISEASE(S): Breast Cancer

SUBMITTER: Rui Branca

LAB HEAD: Janne Lehtiö

PROVIDER: PXD016112 | Pride | 2021-09-10

REPOSITORIES: Pride

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Therapeutic Cancer Vaccination with Immunopeptidomics-Discovered Antigens Confers Protective Antitumor Efficacy.

Peltonen Karita K Feola Sara S Umer Husen M HM Chiaro Jacopo J Mermelekas Georgios G Ylösmäki Erkko E Pesonen Sari S Branca Rui M M RMM Lehtiö Janne J Cerullo Vincenzo V

Cancers 20210707 14

Knowledge of clinically targetable tumor antigens is becoming vital for broader design and utility of therapeutic cancer vaccines. This information is obtained reliably by directly interrogating the MHC-I presented peptide ligands, the immunopeptidome, with state-of-the-art mass spectrometry. Our manuscript describes direct identification of novel tumor antigens for an aggressive triple-negative breast cancer model. Immunopeptidome profiling revealed 2481 unique antigens, among them a novel ERV ...[more]

PMID: 34298622

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Immunopeptidomics of malignant mesothelioma to identify target antigens for T cell Immunotherapy

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Mapping of immunopeptidome of cancer immunotherapy with oncolytic virus and checkpoint blockade

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Antigen Presentation Profiling Reveals T-cell Recognition of Lymphoma Immunoglobulin Neoantigens

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2017-03-27 | PXD005704 | Pride

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The self-peptide repertoire plays a critical role in transplant tolerance induction

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