Dataset Information

No evidence for major spacial differences within the microbial communities in anaerobic digestion plants under different feeding and stirring regimes

ABSTRACT: In anaerobic digestion plants (ADP) homogenization of the feed, the fermenter content and the microbial communities represents a precondition for effective and robust biogas production,but also a major energy consumer. For a 850 m3 agricultural AD-P equipped with eight sampling ports we investigated whether different feeding and stirring regimes enable a sufficient homogenization of the microbial communities using metaproteome and TRFLP analysis. Systematic comparison of the samples by scatter plots and students t-test revealed only a limited number of slightly changed metaproteins, taxonomies and biological processes, indicating no systematic differences between the microbial communities in center and rim as well as between top and bottom. However, comparison of the amount of shared identified metaproteins between the sample ports showed minor variation, which might be correlated with the applied stirring strategy. In sum, the applied stirring and feeding conditionswere sufficient to homogenize the microbial communities in AD-Ps largely.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Robert Heyer

LAB HEAD: Dirk Benndorf

PROVIDER: PXD016213 | Pride | 2021-04-27

REPOSITORIES: Pride

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Impact of feeding and stirring regimes on the internal stratification of microbial communities in the fermenter of anaerobic digestion plants.

Heyer Robert R Klang Johanna J Hellwig Patrick P Schallert Kay K Kress Philipp P Huelsemann Benedikt B Theuerl Susanne S Reichl Udo U Benndorf Dirk D

Bioresource technology 20200613

In anaerobic digestion plants (ADs), homogenization of the feed, fermenter content and microbial communities is crucial for efficient and robust biogas production. However, mixing also requires a significant amount of energy. For an 850 m<sup>3</sup> agricultural AD equipped with eight sampling ports, we investigated whether different feeding and stirring regimes enable a sufficient homogenization of the microbial community using metaproteomics and terminal restriction fragment length polymorphi ...[more]

PMID: 32629381

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			Action	DRS
	Fermenter01.dat	Other
	Fermenter01.mgf	Mgf
	Fermenter01.raw	Raw
	Fermenter02.dat	Other
	Fermenter02.mgf	Mgf