Proteomics

Dataset Information

0

No evidence for major spacial differences within the microbial communities in anaerobic digestion plants under different feeding and stirring regimes


ABSTRACT: In anaerobic digestion plants (ADP) homogenization of the feed, the fermenter content and the microbial communities represents a precondition for effective and robust biogas production,but also a major energy consumer. For a 850 m3 agricultural AD-P equipped with eight sampling ports we investigated whether different feeding and stirring regimes enable a sufficient homogenization of the microbial communities using metaproteome and TRFLP analysis. Systematic comparison of the samples by scatter plots and students t-test revealed only a limited number of slightly changed metaproteins, taxonomies and biological processes, indicating no systematic differences between the microbial communities in center and rim as well as between top and bottom. However, comparison of the amount of shared identified metaproteins between the sample ports showed minor variation, which might be correlated with the applied stirring strategy. In sum, the applied stirring and feeding conditionswere sufficient to homogenize the microbial communities in AD-Ps largely.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli

SUBMITTER: Robert Heyer  

LAB HEAD: Dirk Benndorf

PROVIDER: PXD016213 | Pride | 2021-04-27

REPOSITORIES: Pride

altmetric image

Publications

Impact of feeding and stirring regimes on the internal stratification of microbial communities in the fermenter of anaerobic digestion plants.

Heyer Robert R   Klang Johanna J   Hellwig Patrick P   Schallert Kay K   Kress Philipp P   Huelsemann Benedikt B   Theuerl Susanne S   Reichl Udo U   Benndorf Dirk D  

Bioresource technology 20200613


In anaerobic digestion plants (ADs), homogenization of the feed, fermenter content and microbial communities is crucial for efficient and robust biogas production. However, mixing also requires a significant amount of energy. For an 850 m<sup>3</sup> agricultural AD equipped with eight sampling ports, we investigated whether different feeding and stirring regimes enable a sufficient homogenization of the microbial community using metaproteomics and terminal restriction fragment length polymorphi  ...[more]

Similar Datasets

2018-12-19 | PXD004328 | Pride
2019-05-03 | PXD009349 | Pride
2024-01-26 | PXD044571 | Pride
2019-03-11 | PXD010371 | Pride
2016-04-19 | PXD003526 | Pride
2020-03-02 | PXD010872 | Pride
2023-03-11 | PXD034618 | Pride
2021-06-11 | PXD018503 | Pride
2015-07-21 | PXD001508 | Pride
2015-05-23 | E-GEOD-69171 | biostudies-arrayexpress