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FACS sorted podocytes, mesangial cells

ABSTRACT: FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD016237 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Tripartite Separation of Glomerular Cell Types and Proteomes from Reporter-Free Mice.

Hatje Favian A FA Wedekind Uta U Sachs Wiebke W Loreth Desiree D Loreth Desiree D Reichelt Julia J Demir Fatih F Kosub Christopher C Heintz Lukas L Tomas Nicola M NM Huber Tobias B TB Skuza Sinah S Sachs Marlies M Zielinski Stephanie S Rinschen Markus M MM Meyer-Schwesinger Catherine C

Journal of the American Society of Nephrology : JASN 20210601 9

<h4>Background</h4>The glomerulus comprises podocytes, mesangial cells, and endothelial cells, which jointly determine glomerular filtration. Understanding this intricate functional unit beyond the transcriptome requires bulk isolation of these cell types for biochemical investigations. We developed a globally applicable tripartite isolation method for murine mesangial and endothelial cells and podocytes (timMEP).<h4>Methods</h4>We separated glomerular cell types from wild-type or mT/mG mice <i> ...[more]

PMID: 34074698

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