Proteomics

Dataset Information

0

Mapping Axon Initial Segment Structure and Function by Iterative Proximity Biotinylation

ABSTRACT: We constructed AIS-targeted biotin ligase by generating fusion proteins of BirA* with NF186, Ndel1, and Trim46; these chimeras mapped the molecular organization of intracellular membrane, cytosolic, and microtubule compartments of the AIS, respectively

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Juan Oses-Prieto  

LAB HEAD: Alma L Burlingame

PROVIDER: PXD016272 | Pride | 2020-01-13

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Axon initial segments (AISs) generate action potentials and regulate the polarized distribution of proteins, lipids, and organelles in neurons. While the mechanisms of AIS Na<sup>+</sup> and K<sup>+</sup> channel clustering are understood, the molecular mechanisms that stabilize the AIS and control neuronal polarity remain obscure. Here, we use proximity biotinylation and mass spectrometry to identify the AIS proteome. We target the biotin-ligase BirA* to the AIS by generating fusion proteins of  ...[more]

Similar Datasets

Proteomics Immunoproximity biotinylation reveals the axon initial segment proteome
2024-01-02 | PXD045921 | Pride
Unknown Mapping axon initial segment structure and function by multiplexed proximity biotinylation.
| S-EPMC6941957 | biostudies-literature
Proteomics Analysis of NF186 proximity proteomes across neuronal development using Nfasc antibodies in an antibody-directed proximity biotinylation strategy combined with mass spectrometry
2023-10-05 | PXD043805 | Pride
Unknown Neuron Morphology Influences Axon Initial Segment Plasticity.
| S-EPMC4756267 | biostudies-literature
Unknown Feedback-Driven Assembly of the Axon Initial Segment.
| S-EPMC6839619 | biostudies-literature
Unknown Modeling tau transport in the axon initial segment.
| S-EPMC8550824 | biostudies-literature
Unknown Inhibitory Synapse Formation at the Axon Initial Segment.
| S-EPMC6848228 | biostudies-literature
Unknown Theoretical relation between axon initial segment geometry and excitability.
| S-EPMC7170651 | biostudies-literature
Unknown Axon initial segment geometry in relation to motoneuron excitability.
| S-EPMC8604372 | biostudies-literature
Unknown TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment.
| S-EPMC6670255 | biostudies-literature