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Identification of flg22-responsive BSU1 phosphorylation sites

ABSTRACT: To investigate flg22-induced posttranslational modification of BSU1 family proteins, we performed metabolic Stable Isotope Labeling In Arabidopsis followed by Immuno-Precipitation and Mass Spectrometry (SILIA IP-MS) analysis, with the isotopes switched in replicate experiments.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Shouling Xu

LAB HEAD: Shouling Xu

PROVIDER: PXD016283 | Pride | 2023-03-10

REPOSITORIES: Pride

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			Action	DRS
	V20140825-07.raw	Raw
	V20140825-07_ITMSms2cid.pep.xml	Pepxml

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Publications

Deconvoluting signals downstream of growth and immune receptor kinases by phosphocodes of the BSU1 family phosphatases.

Park Chan Ho CH Bi Yang Y Youn Ji-Hyun JH Kim So-Hee SH Kim Jung-Gun JG Xu Nicole Y NY Shrestha Ruben R Burlingame Alma L AL Xu Shou-Ling SL Mudgett Mary Beth MB Kim Seong-Ki SK Kim Tae-Wuk TW Wang Zhi-Yong ZY

Nature plants 20220613 6

Hundreds of leucine-rich repeat receptor kinases (LRR-RKs) have evolved to control diverse processes of growth, development and immunity in plants, but the mechanisms that link LRR-RKs to distinct cellular responses are not understood. Here we show that two LRR-RKs, the brassinosteroid hormone receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) and the flagellin receptor FLAGELLIN SENSING 2 (FLS2), regulate downstream glycogen synthase kinase 3 (GSK3) and mitogen-activated protein (MAP) kinases, respe ...[more]

PMID: 35697730

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SPY mediates O-fucose modification of hundreds of proteins and sugar-dependent growth in Arabidopsis_(Part 1: HCD)

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Quantitative proteomic comparison between wild type Col and acinus-2 pinin-1 mutant using 15N metabolic labeling

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Phylogenetic patterns in the microbial response to resource availability: amino acid incorporation in San Francisco Bay

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