Proteomics

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Basic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion


ABSTRACT: Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established. Although great progresses have been continually achieved, it is still nec-essary to develop more efficient approaches in order to discover a proteome-scale protein C-termini (C-terminome), and consequently to help understand their biological functions. In this report, we describe a simple method, termed BaSCX, for basic strong cation exchange chromatography, to study C-terminome.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human) Caenorhabditis Elegans Mus Musculus (mouse)

TISSUE(S): Cell Culture, Whole Body

SUBMITTER: Qingqing Li  

LAB HEAD: Xumin Zhang

PROVIDER: PXD016317 | Pride | 2020-02-28

REPOSITORIES: Pride

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Publications

Basic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion.

Li Qingqing Q   Zhang Yang Y   Huang Jingnan J   Wu Zhen Z   Tang Langlang L   Huang Lin L   Zhang Xumin X  

Analytical chemistry 20200309 7


Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical and enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS)-based protein C-termini analysis approaches have been established. Although great progress is being continually achieved, it is still necessary to develop more efficient approaches in order to discover a proteome-scale protein C-termini (C-terminome) and consequently to help underst  ...[more]

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