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Yeast proteomic analysis upon temperature shift

ABSTRACT: DIA/SWATH analysis of yeast upon temperature shift.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Ludovic Gillet

LAB HEAD: Ruedi A Aebersold

PROVIDER: PXD016320 | Pride | 2021-06-28

REPOSITORIES: Pride

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Puf6 primes 60S pre-ribosome nuclear export at low temperature.

Gerhardy Stefan S Oborská-Oplová Michaela M Gillet Ludovic L Börner Richard R van Nues Rob R Leitner Alexander A Michel Erich E Petkowski Janusz J JJ Granneman Sander S Sigel Roland K O RKO Aebersold Ruedi R Panse Vikram Govind VG Panse Vikram Govind VG

Nature communications 20210804 1

Productive ribosomal RNA (rRNA) compaction during ribosome assembly necessitates establishing correct tertiary contacts between distant secondary structure elements. Here, we quantify the response of the yeast proteome to low temperature (LT), a condition where aberrant mis-paired RNA folding intermediates accumulate. We show that, at LT, yeast cells globally boost production of their ribosome assembly machinery. We find that the LT-induced assembly factor, Puf6, binds to the nascent catalytic R ...[more]

PMID: 34349113

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