Purification of proteasome-interacting proteins for MS analysis.
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Colon
SUBMITTER: 
Hikaru Tsuchiya
LAB HEAD: Yasushi Saeki
PROVIDER: PXD016367 | Pride | 2026-03-20
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| XlinkMS_PSMD6-KI__20160314_.mgf | Mgf | |||
| XlinkMS_PSMD6-KI__20160314_.msf | Msf | |||
| XlinkMS_PSMD6-KI__20160314_.raw | Raw | |||
| XlinkMS_WT__20160314_.mgf | Mgf | |||
| XlinkMS_WT__20160314_.msf | Msf |
Items per page: 5 1 - 5 of 7 |
Similar Datasets
Project description:By hyperosmotic stimuli, a slight increase in the level of ubiquitylated proteins was observed. So we tried to identify the di-Gly peptides.
2020-02-18 | PXD016369 | Pride
Project description:Quantification of ubiquitin chain linkage of uS10. Quantification of ubiquitin chain linkage of uS10in UbiR54A mutant strain.
2025-09-18 | PXD052346 | Pride
Project description:Bortezomib is a proteasome inhibitor used in severel different hematological malignancies. Resistance to this drug is still poorly understood. In order get more insight in the resistance mechanism, we developed several bortezomib resistant subclones of the THP-1 monocytic/macrophage cell line. On these subclones expression arrays were performed. We performed expression array three different bortezomib resistant subclones of the THP-1 cell line. The resistant subclones were spotted against the parental THP-1 wildtype cell line.
2008-06-14 | E-GEOD-11771 | biostudies-arrayexpress
Project description:Multiple Myeloma (MM) is cancer in the antibody-producing plasma cells. It comprises 1 percent of all hematological malignancies. MM is incurable and fatal. The proteasome inhibitor bortezomib has improved treatment significantly, but inherent and acquired resistance remains a problem. Glutathione (GSH) is an important red-ox buffer in eukaryotic cells. In this experiment we investigate how GSH affects bortezomib-induced gene expression changes
2014-08-01 | E-MTAB-2666 | biostudies-arrayexpress
Project description:In comparison with allogeneic hematopoietic stem cell transplantation using other sources, umbilical cord blood transplantation (UCBT) offers substantial clinical advantages including a lower incidence and severity of acute graft-versus-host disease (GVHD) despite the use of allogeneic UCB stem cells with more disparate HLA. However, detailed pathophysiology of acute GVHD developed after UCBT has not yet been clarified. Here, we examined the roles of inflammatory cells in the pathogenesis of acute GVHD following UCBT, by expression profiling of a total of 595 genes in each of 4 subsets (CD4+, CD8+, CD14+, and CD56+) of peripheral blood mononuclear cells (PBMCs), which were taken from 3 patients with hematologic malignancy, in whom acute GVHD had developed after UCBT. We compared expression profiles measured during acute GVHD with those measured opon discharge (recovery phase), for each subset of PBMCs to identify immuno-regulatory genes which were assumed to be linked to the pathogenesis of acute GVHD. Keywords: disease state analysis Peripheral blood mononuclear cells (PBMCs) were taken from 3 patients with hematologic malignancy, in whom acute GVHD had developed after cord blood transplantation. CD4+, CD8+, CD14+, and CD56+ cell subsets were isolated from the PBMCs of 2 patients, while only CD8+ and CD56+ cell subsets were isolated from the PBMCs of a patient. PBMCs were taken twice - during acute GVHD phase and upon discharge (recovery phase), from each patient. By using a custom-made fibrous oligonucleotide DNA microarray Genopal (Mitsubishi Rayon), we compared expression profiles of 595 unique genes measured during acute GVHD with those measured upon discharge (recovery phase), for each subset of PBMCs.
2010-05-15 | E-GEOD-10512 | biostudies-arrayexpress
Project description:Stable inheritance of DNA methylation is critical for maintaining the differentiated phenotypes in multicellular organisms. However, the molecular basis ensuring high fidelity of maintenance DNA methylation is largely unknown. Here, we demonstrate that two distinct modes of DNMT1 recruitment, one is DNA replication-coupled and the other is uncoupled mechanism, regulate the stable inheritance of DNA methylation. PCNA-associated factor 15 (PAF15) represents a primary target of UHRF1 and undergoes dual mono-ubiquitylation (PAF15Ub2) on chromatin. PAF15Ub2 specifically interacts with DNMT1 and controls the recruitment of DNMT1 in a DNA replication-coupled manner. Thus, loss of PAF15Ub2 results in impaired DNA methylation at sites replicating during early S phase. In contrast, outside of S phase or when PAF15 ubiquitylation is perturbed, UHRF1 ubiquitylates histone H3 to promote DNMT1 recruitment. Together, we identify replication-coupled and uncoupled mechanisms of maintenance DNA methylation, both of which collaboratively ensure the stable DNA methylation.
2020-02-25 | PXD015282 | Pride
Project description:Bromine is the most common organohalide found in nature. This halogen is often produced from sea brines and salt lakes. The primary producers and consuming countries of Br2 include the USA, Israel, Jordon, and China. The production of many products including brominated fire retardants (BFR’s), in addition to drilling fluids, agricultural materials, and biocides for the treatment of water all require Br2. While accidental exposure has occurred during production processes and transportation, a major concern is that larger storehouses of Br2 are potential targets for terrorist groups throughout the World. Higher concentrations of Br2 are life threatening within as little as 30 minutes. In our present study, we utilized the common C57BL/6 mouse model to survey the lung and serologic proteomes following exposure to near lethal levels of Br2 for 30 minutes. We describe detailed acute changes found within the global proteomes of these animals, in addition to post-translationally modified brominated proteins longitudinally over three time points from 30min to 96hrs post exposure. Through the application of common proteomics workflows combined with systems biology analysis, we were able to identify robust and highly significant biomarkers and pathways associated with Br2 exposure that are new to this model.
2020-06-18 | PXD015462 | Pride
Project description:This SuperSeries is composed of the following subset Series: GSE10512: Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.1) GSE10513: Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.2) Keywords: SuperSeries Refer to individual Series
2008-12-21 | E-GEOD-10572 | biostudies-arrayexpress
Project description:Ten-eleven translocation-2 (TET2) is a member of the methylcytosine dioxygenase family of enzymes implicated in cancer and in aging due to its role as a global epigenetic modifier. TET2 has a large N-terminal domain followed by a catalytic C-terminal. Previous reports have demonstrated that the catalytic domain remains active independent of the N-terminal domain. As such, the function of the N-terminus of this large protein remains poorly characterized. Here, we identify that several isoforms of the 14-3-3 family of proteins bind TET2. 14-3-3s bind TET2 when phosphorylated at serine 99 (S99). AMPK-mediated phosphorylation at S99 promotes TET2 stability and increases global DNA 5-hydroxymethylcytosine. 14-3-3s’ interaction with TET2 serves to protect S99 phosphorylation. Disruption of this interaction leads to both reduced TET2 phosphorylation and decreased protein stability. Furthermore, we identify that the protein phosphatase 2A (PP2A) can interact with TET2 and dephosphorylates S99. Collectively, our study provides novel insights into the role of the N-terminal domain in TET2 regulation. Moreover, they demonstrate the dynamic nature of TET2 protein regulation that could have therapeutic implications for disease states resulting from reduced TET2 levels and/or activity.
2020-01-13 | PXD016160 | Pride
Project description:To explore the possible neddylation sites, GFP-IRF7 and His-NEDD8 were co-overexpressed in HEK293 cell line and GFP was immunoprecipitated, followed by SDS-PAGE and silver staining. The band detected was subjected to LC–MS/MS.
2021-08-19 | PXD024052 | Pride