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Salmonella Typhimurium SL1344 proteogenomics

ABSTRACT: We describe how searching chimeric spectra with post-processing including MS²PIP-derived features aids the identification of hypothetical and unannotated proteins. We apply our workflow to the well-characterized human bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) and validate novel protein-coding regions with by ribo-seq translation evidence. We further elaborate how riboproteogenomics is instrumental for reannotating ORFs and the discovery of novel ORFs across bacteria.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344

TISSUE(S): Cell Culture

SUBMITTER: Patrick Willems

LAB HEAD: Petra Van Damme

PROVIDER: PXD016377 | Pride | 2020-10-13

REPOSITORIES: Pride

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